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The DNA fragments produced by the use of restriction endonucleases can be separated by
General Science Biology Class 12th NEET UG NEET UG Preparation
Q. 3 of Quiz-1461 (Que-ID : 3396)
The DNA fragments produced by the use of restriction endonucleases can be separated by
- polymerase chain reaction
- gel electrophoresis
- density gradient centrifugation
- any of the above
Ans → (b)gel electrophoresis
Explanation → The DNA fragments produced by the use of restriction endonucleases can be separated by gel electrophoresis
This question is taken from this post → Biotechnology: Principles and Processes - Biology (Class 12th) Revision Test | MCQs for NEET - attemp/practice
More MCQs in - Biotechnology: Principles and Processes - Biology (Class 12th) Revision Test | MCQs for NEET
Q. 1 of Quiz-1461 (Que-ID : 3394)
Biolistics (gene gun) is suitable for
- introducing rDNA into plant cells
- introducing rDNA into animal cells
- disarming the pathogen vectors
- DNA fingerprinting.
Q. 2 of Quiz-1461 (Que-ID : 3395)
In genetic engineering experiments, restriction enzymes are used for
- viral DNA
- bacterial DNA
- eukaryotic DNA
- any type of DNA.
Q. 3 of Quiz-1461 (Que-ID : 3396)
The DNA fragments produced by the use of restriction endonucleases can be separated by
- polymerase chain reaction
- gel electrophoresis
- density gradient centrifugation
- any of the above
Q. 4 of Quiz-1461 (Que-ID : 3397)
Plasmids in bacterial cells are
- extra-chromosomal DNA, which cannot replicate
- extra-chromosomal DNA, which can . self-replicate
- extra DNA associated with the genome
- extra DNA, associated with the genome, but cannot replicate.
Q. 5 of Quiz-1461 (Que-ID : 3398)
The DNA polymerase enzyme used in PCR is obtained from
- Thermus aquaticus
- Escherichia coli
- Agrobacterium tumefaciens
- Salmonella typhimurium
Q. 6 of Quiz-1461 (Que-ID : 3399)
While isolating DNA from bacteria, which of the following enzymes is not used?
- Lysozyme
- Ribonuclease
- Deoxyribonuclease
- Protease
Q. 7 of Quiz-1461 (Que-ID : 3400)
Significance of ‘heat shock’ method in bacterial transformation is to facilitate
- binding of DNA to the cell wall
- uptake of DNA through membrane transport proteins
- uptake of DNA through transient pores in the bacterial cell wall
- expression of antibiotic resistance gene
Q. 8 of Quiz-1461 (Que-ID : 3401)
Which of the following steps are catalysed by Taq polymerase in a PCR reaction?
- Denaturation of template DNA
- Annealing of primers to template DNA
- Extension of primer end on the template DNA
- All of the above
Q. 9 of Quiz-1461 (Que-ID : 3402)
Which of the given statement is correct in the context of observing DNA separated by agarose gel electrophoresis?
- DNA can be seen in visible light
- DNA can be seen without staining in visible light
- Ethidium bromide stained DNA can be seen in visible light
- Ethidium bromide stained DNA can be seen under exposure to UV light
Q. 10 of Quiz-1461 (Que-ID : 3403)
Restriction’ in Restriction enzyme refers to
- cleaving ofphosphodiester bond in DNA by the enzyme
- cutting of DNA at specific position only
- prevention of the multiplication of bacteriophage in bacteria
- all of the above
Q. 11 of Quiz-1461 (Que-ID : 3404)
The role of DNA ligase in the construction of a recombinant DNA molecule is
- formation of phosphodiester bond between two DNA fragments
- formation of hydrogen bonds between sticky ends of DNA fragments
- ligation of all purine and pyrimidine bases
- none of the above
Q. 12 of Quiz-1461 (Que-ID : 3405)
In an experiment, recombinant DNA bearing ampicillin-resistance gene is transferred into E.coli cells. The host cells are then cultured on a medium containing ampicillin. The result will be
- both transformants and non-transformants cannot survive
- both transformants and non-transformants can survive.
- transformants only and not the non-transformants can survive
- transformants cannot survive, but non-transformants can not.
Q. 13 of Quiz-1461 (Que-ID : 3406)
DNA element with ability to change its position is called
- Cistron
- Transposon
- Intron
- Ricon
Q. 14 of Quiz-1461 (Que-ID : 3407)
Viral genome incroporatcd into host DNA is called:
- Prophase
- Protophage
- Bacteriophage
- None of these
Q. 15 of Quiz-1461 (Que-ID : 3408)
Triticale, first man made cereal crop, has been obtained by crossing wheat with:
- Rye
- Pearl millet
- Sugarcane
- Barley
Q. 16 of Quiz-1461 (Que-ID : 3409)
Golden rice is a promising transgenic crop. When released for cultivation, it will help in :
- High lysin content
- Pest resistance
- High protien content
- High vitamin A content
Q. 17 of Quiz-1461 (Que-ID : 3410)
Restrictipn endonucleases are most widely used in recombinant DNA technology. They are obtained from
- Plasmids
- AH prokaryotic cells
- Bacteriophages
- Bacterial cells
Q. 18 of Quiz-1461 (Que-ID : 3411)
Restriction endonuclease cuts
- One stand of DNA at specific site
- Both strands of DNA
- Both strands of DNA at any site
- Single strand of RNA
Q. 19 of Quiz-1461 (Que-ID : 3412)
Plasmid is a
- Fungus
- Plasmid
- Part of Plasma membrane
- Extra chromosomal DNA in bacterial cell
Q. 20 of Quiz-1461 (Que-ID : 3413)
Two microbes found tb be very useful in Genetic Engineering are
- Excherichia coli and Agrobacterium tumefaciens
- Vibrio cholarae and a tailed bacteriophage
- Diplococcus sp. and Pseudomonas sp.
- Crown gall bacterium and Caenorhabdities elegans
Q. 21 of Quiz-1461 (Que-ID : 3414)
DNA obtained for DNA finger printing:
- W.B.C
- Hair root cells
- Body fluids
- None of these
Q. 22 of Quiz-1461 (Que-ID : 3415)
Who discovered Restricted endonucleas enzymes?
- Smith and Nathane
- Baiger
- Waxman
- Fleming
Q. 23 of Quiz-1461 (Que-ID : 3416)
Two microbes found to be very useful in genetic, engineering are
- Escherichiya Coli and Agrobacterium tumefaciens
- Virbio chloerae and tailed bacteriophage
- Diplococcus sp and pseudomonas sp
- None of these
Q. 24 of Quiz-1461 (Que-ID : 3417)
Transgenic plants are develop by
- Introducing of foreign fenes
- Clone and genetically modified genes
- Genetic engineering
- Purified genes
Q. 25 of Quiz-1461 (Que-ID : 3418)
Transgenic plants are develop by
- Introducing of foreign fenes
- Clone and genetically modified genes
- Genetic engineering
- Purified genes
More MCQs In - General Science Biology Class 12th NEET UG NEET UG Preparation
Q. 1 of Quiz- (Que-ID : 3069)
The rate of photosynthesis is controlled by
- the rate of light reaction
- the rate of dark reaction
- the rates of both light and dark reactions
- none of the above
Q. 8 of Quiz- (Que-ID : 2876)
From the sexually transmitted diseases mentioned below, identify the one which does not specifically affect the sex organs
- Syphilis
- AIDS
- Gonorrhea
- Genital warts
Q. 14 of Quiz- (Que-ID : 2807)
Which of the following is not related to the autonomic nervous system?
- Peristalsis
- Digestion
- Excretion
- Memory and learning
Q. 22 of Quiz- (Que-ID : 3515)
Which group of vertebrates comprises the highest number of endangered species?
- Fishes
- Reptiles
- Birds
- Mammals
Q. 13 of Quiz- (Que-ID : 2831)
The male gametes of rice plant have 12 chromosomes in their nucleus. The chromosome number in the female gamete, zygote and the cells of the seedling will be, respectively
- 12, 24, 12
- 24, 12, 12
- 12, 24, 24
- 24, 12, 24
Q. 17 of Quiz- (Que-ID : 2910)
spot the odd one out from the following structures with reference to the male reproductive system
- Rate testis
- Epididymis
- Vasa efferentia
- Isthmus
Q. 9 of Quiz- (Que-ID : 3327)
Hepatitis B is transmitted through
- sneezing
- female Anopheles
- coughing
- blood transfusion
Q. 23 of Quiz- (Que-ID : 3091)
Structurally, chlorophyll a and b are different as
- Chl a has a methyl group and Chl b has an aldehyde group.
- Chl a has an aldehyde group and Chl b has a methyl group.
- Chl a has an ethyl group and Chl b has an aldehyde group
- Chl a has a carboxyl group and Chl b has an aldehyde group.
Q. 22 of Quiz- (Que-ID : 3465)
Animals undergo inactive stage during winters. It is called
- Acclimatisation
- Hibernation
- Aestivation
- Adapatation
Q. 13 of Quiz- (Que-ID : 3081)
Where are thylakoids and grana located?
- Lysosomes
- Mitochondria
- Chloroplasts
- Golgi apparatus
Q. 2 of Quiz- (Que-ID : 2745)
Secretin cause stimulation of
- Bile juice
- Water
- Gastric secretion
- Pepsinogen
Q. 9 of Quiz- (Que-ID : 2827)
In flowering plants, both male and female gametes are non-motile. The method to bring them together for fertilisation is
- water
- air
- pollination
- apomixis
Q. 22 of Quiz- (Que-ID : 3015)
Division of nucleus without being followed by cytokinesis results into
- Phargmoplast
- Polyploidy
- Uninucleate condition
- Multinucleate condition
Q. 10 of Quiz- (Que-ID : 3553)
Which of the following quantum number is also known as orbital angular momentum?
- Magnetic quantum number
- Principal quantum number
- Azimuthal quantum number
- Electron spin quantum number
Q. 18 of Quiz- (Que-ID : 2811)
The thin and convoluted outer layer of gray matter that covers the cerebral hemispheres is
- Medulla oblongata
- Thalamus
- Cerebral cortex
- Meninges
Q. 6 of Quiz- (Que-ID : 2619)
Which cell is responsible for secretion of HCL and intrinsic factors
- Parietal cell
- Chief cell
- Mucus cell
- Peptic cell
Q. 2 of Quiz- (Que-ID : 3445)
The size of the population tells us a lot about its status in the
- Environment
- Sex-ratio
- Age pyramid
- Habitat
Q. 15 of Quiz- (Que-ID : 3508)
Identify the correct matched pair
- Montreal Protocol-Global Warming
- Kyoto Protocol-Climate change
- Project Hungul-Dear
- Project Tiger-Lion
Q. 8 of Quiz- (Que-ID : 3426)
In a Transgenic crop gene is for the following:
- New protein synthesis
- Resistance from Antibiotics
- In the formation enzyme for Antibiotics
- All of these
Q. 20 of Quiz- (Que-ID : 2788)
Which of the following is correct?
- Musca domestica Order ? Diptera Family ? Muscidae Phylum ? Arthropoda
- Mangifera indica Order ? Sapindales Family ? Anacardiaceae Phylum ? Gymnospermae
- Triticum aestivum Order ? Poles Family ? Monocotyledons Phylum ? Angiospermae
- Panthera leo Order ? leo Family ? Felidae Phylum ? Mammalia
Q. 3 of Quiz- (Que-ID : 3221)
Exceptional roots of Cuscuta are
- haustorial
- coralloid
- mucorhizal
- all of the above
Q. 11 of Quiz- (Que-ID : 2904)
Preparation of sperm before penetration of ovum is
- spermiation
- cortical reaction
- spermiogenesis
- capacitation
Q. 8 of Quiz- (Que-ID : 3351)
Continued inbreeding, especially close inbreeding generally results in
- inbreeding depression
- inbreeding stimulation
- inbreeding hybridisation
- inbreeding mutation
Q. 22 of Quiz- (Que-ID : 3465)
Animals undergo inactive stage during winters. It is called
- Acclimatisation
- Hibernation
- Aestivation
- Adapatation
Q. 23 of Quiz- (Que-ID : 3091)
Structurally, chlorophyll a and b are different as
- Chl a has a methyl group and Chl b has an aldehyde group.
- Chl a has an aldehyde group and Chl b has a methyl group.
- Chl a has an ethyl group and Chl b has an aldehyde group
- Chl a has a carboxyl group and Chl b has an aldehyde group.
Q. 12 of Quiz- (Que-ID : 3530)
Which one of the following is not a greenhouse gas?
- Methane
- CO2
- Chlorofluoro carbon
- Hydrogen
Q. 25 of Quiz- (Que-ID : 2918)
At what stage of life is oogenesis initiated in a human female ?
- At puberty
- During menarch
- During menopause
- During embryonic development
Q. 14 of Quiz- (Que-ID : 2732)
Heterocyst in nostoc participates in
- Nitrogen fixation
- Fragmentation
- Fruit storage
- Symbiotic relation
Q. 14 of Quiz- (Que-ID : 3382)
During the primary treatment of sewage, solid particles that settle down are called
- floes
- primary sludge
- activated sludge
- anaerobic sludge
Q. 3 of Quiz- (Que-ID : 2996)
Choose the correct sequence. A. Chromatin condensation B. Protein synthesis C. Duplication of centrioles D. Centrioles moves towards opposite poles
- C, A, B, D
- C, B, A, D
- C, D, B, A
- A, C, D, B
Q. 14 of Quiz- (Que-ID : 3307)
The Hardy-Weinberg principle cannot operate if
- a population does not migrate for a longtime to a new habitat
- frequent mutations occur in the population
- the population has no chance of interaction with other populations
- free interbreeding occurs among all members of the population
Q. 9 of Quiz- (Que-ID : 3077)
Besides water and CO2, which is more essential a raw material for food formation
- light
- oxygen
- NAD
- mineral salt
Q. 8 of Quiz- (Que-ID : 2676)
Which among the following WBC secrete histamine, serotonin,heparin
- Eosinophil
- Neutrophil
- Basophil
- None of the above
Q. 12 of Quiz- (Que-ID : 2655)
which among the following is measured by spirometer
- Total lung capacity
- FRC
- RV
- TV
Q. 11 of Quiz- (Que-ID : 1860)
Which of the following was the first to impose censorship of the press?
- Wellesley
- Hastings
- Johan Adams
- Dalhousie
Q. 8 of Quiz- (Que-ID : 3476)
Small extremite animals of tropical region
- Allen’s law
- Bergmen’s law
- Dollo’s law
- Cope’s law
Q. 19 of Quiz- (Que-ID : 3312)
presence of gills in the tadpole of frog indicated that
- fishes were amphibious in the past
- fishes evolved from frog-like ancestors
- frogs will have gills in future
- frogs evolved from gilled ancestors
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