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Q.18. Restriction endonuclease cuts

  1. One stand of DNA at specific site
  2. Both strands of DNA
  3. Both strands of DNA at any site
  4. Single strand of RNA
Answer: (b) Both strands of DNA
Explanation: Restriction endonuclease cuts both strands of DNA

Biotechnology: Principles And Processes - Biology (Class 12th) Revision Test | MCQs For NEET

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This practice test consists of 25 important questions from the Biology Chapter - Biotechnology: Principles And Processes (Class 12). these questions and practice test is helpful for NEET Ug and other competitive entrance exams. Start Test without Login in
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Q.1. Biolistics (gene gun) is suitable for

  1. introducing rDNA into plant cells
  2. introducing rDNA into animal cells
  3. disarming the pathogen vectors
  4. DNA fingerprinting.

Q.2. In genetic engineering experiments, restriction enzymes are used for

  1. viral DNA
  2. bacterial DNA
  3. eukaryotic DNA
  4. any type of DNA.

Q.3. The DNA fragments produced by the use of restriction endonucleases can be separated by

  1. polymerase chain reaction
  2. gel electrophoresis
  3. density gradient centrifugation
  4. any of the above

Q.4. Plasmids in bacterial cells are

  1. extra-chromosomal DNA, which cannot replicate
  2. extra-chromosomal DNA, which can . self-replicate
  3. extra DNA associated with the genome
  4. extra DNA, associated with the genome, but cannot replicate.

Q.5. The DNA polymerase enzyme used in PCR is obtained from

  1. Thermus aquaticus
  2. Escherichia coli
  3. Agrobacterium tumefaciens
  4. Salmonella typhimurium

Q.6. While isolating DNA from bacteria, which of the following enzymes is not used?

  1. Lysozyme
  2. Ribonuclease
  3. Deoxyribonuclease
  4. Protease

Q.7. Significance of ‘heat shock’ method in bacterial transformation is to facilitate

  1. binding of DNA to the cell wall
  2. uptake of DNA through membrane transport proteins
  3. uptake of DNA through transient pores in the bacterial cell wall
  4. expression of antibiotic resistance gene

Q.8. Which of the following steps are catalysed by Taq polymerase in a PCR reaction?

  1. Denaturation of template DNA
  2. Annealing of primers to template DNA
  3. Extension of primer end on the template DNA
  4. All of the above

Q.9. Which of the given statement is correct in the context of observing DNA separated by agarose gel electrophoresis?

  1. DNA can be seen in visible light
  2. DNA can be seen without staining in visible light
  3. Ethidium bromide stained DNA can be seen in visible light
  4. Ethidium bromide stained DNA can be seen under exposure to UV light

Q.10. Restriction’ in Restriction enzyme refers to

  1. cleaving ofphosphodiester bond in DNA by the enzyme
  2. cutting of DNA at specific position only
  3. prevention of the multiplication of bacteriophage in bacteria
  4. all of the above

Q.11. The role of DNA ligase in the construction of a recombinant DNA molecule is

  1. formation of phosphodiester bond between two DNA fragments
  2. formation of hydrogen bonds between sticky ends of DNA fragments
  3. ligation of all purine and pyrimidine bases
  4. none of the above

Q.12. In an experiment, recombinant DNA bearing ampicillin-resistance gene is transferred into E.coli cells. The host cells are then cultured on a medium containing ampicillin. The result will be

  1. both transformants and non-transformants cannot survive
  2. both transformants and non-transformants can survive.
  3. transformants only and not the non-transformants can survive
  4. transformants cannot survive, but non-transformants can not.

Q.13. DNA element with ability to change its position is called

  1. Cistron
  2. Transposon
  3. Intron
  4. Ricon

Q.14. Viral genome incroporatcd into host DNA is called:

  1. Prophase
  2. Protophage
  3. Bacteriophage
  4. None of these

Q.15. Triticale, first man made cereal crop, has been obtained by crossing wheat with:

  1. Rye
  2. Pearl millet
  3. Sugarcane
  4. Barley

Q.16. Golden rice is a promising transgenic crop. When released for cultivation, it will help in :

  1. High lysin content
  2. Pest resistance
  3. High protien content
  4. High vitamin A content

Q.17. Restrictipn endonucleases are most widely used in recombinant DNA technology. They are obtained from

  1. Plasmids
  2. AH prokaryotic cells
  3. Bacteriophages
  4. Bacterial cells

Q.18. Restriction endonuclease cuts

  1. One stand of DNA at specific site
  2. Both strands of DNA
  3. Both strands of DNA at any site
  4. Single strand of RNA

Q.19. Plasmid is a

  1. Fungus
  2. Plasmid
  3. Part of Plasma membrane
  4. Extra chromosomal DNA in bacterial cell

Q.20. Two microbes found tb be very useful in Genetic Engineering are

  1. Excherichia coli and Agrobacterium tumefaciens
  2. Vibrio cholarae and a tailed bacteriophage
  3. Diplococcus sp. and Pseudomonas sp.
  4. Crown gall bacterium and Caenorhabdities elegans

Q.21. DNA obtained for DNA finger printing:

  1. W.B.C
  2. Hair root cells
  3. Body fluids
  4. None of these

Q.22. Who discovered Restricted endonucleas enzymes?

  1. Smith and Nathane
  2. Baiger
  3. Waxman
  4. Fleming

Q.23. Two microbes found to be very useful in genetic, engineering are

  1. Escherichiya Coli and Agrobacterium tumefaciens
  2. Virbio chloerae and tailed bacteriophage
  3. Diplococcus sp and pseudomonas sp
  4. None of these

Q.24. Transgenic plants are develop by

  1. Introducing of foreign fenes
  2. Clone and genetically modified genes
  3. Genetic engineering
  4. Purified genes

Q.25. Transgenic plants are develop by

  1. Introducing of foreign fenes
  2. Clone and genetically modified genes
  3. Genetic engineering
  4. Purified genes
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